The Effect of Ethanol Extract of Miana Leaves (Plectranthus scutellarioides (L.) Benth.) on the Viability of Human Gingival Fibroblasts
Abstract
Miana leaves (Plectranthus scutellarioides (L.) Benth.) are herbal plants containing bioactive compounds such as flavonoids, tannins, saponins, and alkaloids that potentially exhibit antibacterial and anti-inflammatory activities in dentistry. However, the safety of herbal materials on normal cells needs to be evaluated, including gingival fibroblasts which play an important role in wound healing and tissue regeneration. To determine the effect of ethanol extract of miana leaves on the viability of human gingival fibroblasts at various concentrations and to review its potential utilization from an Islamic perspective. This study was an in vitro experimental laboratory study. Human gingival fibroblast cells were cultured using DMEM medium and treated with ethanol extract of miana leaves at concentrations of 12.5, 25, 50, 100, and 200 µg/mL. The control groups consisted of solvent control, untreated control, and 10% DMSO control. Cell viability was assessed using the Cell Counting Kit-8 (CCK-8) method. Data were analyzed using Kruskal–Wallis test, Post Hoc Mann–Whitney test, and Spearman correlation analysis. The mean viability of gingival fibroblasts at concentrations of 12.5, 25, 50, 100, and 200 µg/mL were 121.48%, 116.69%, 117.09%, 117.09%, and 113.35%, respectively. The Kruskal–Wallis test showed a significant difference between the treatment and control groups (p0.05). There were differences in the viability of human gingival fibroblasts after treatment with ethanol extract of miana leaves, with a tendency for cell viability to decrease as the extract concentration increased.
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References
Aji, N. P., Noviyanti, Y., & Pratiwi, I. (2023). Uji efektivitas ekstrak etanol daun miana (Coleus scutellarioides L) terhadap penyembuhan luka sayat pada kelinci (Oryctolagus cuniculus). Jurnal Ilmiah Pharmacy, 10(2).
Alamgir, A. N. M. (2017). Therapeutic use of medicinal plants and their extracts: Volume 1 (Vol. 73). Springer.
Bismelah, N. A., Ahmad, R., Mohamed Kassim, Z. H., Ismail, N. H., & Rasol, N. E. (2022). The antibacterial effect of Plectranthus scutellarioides (L.) R.Br. leaves extract against bacteria associated with peri-implantitis. Journal of Traditional and Complementary Medicine, 12(6), 556–566.
Cheng, W., Yang, X., Xue, H., Huang, D., Cai, M., Huang, F., Zheng, L., Yu, Z., & Zhang, J. (2022). Reproductive toxicity of furfural acetone in Meloidogyne incognita and Caenorhabditis elegans. Cells, 11(3), 401. https://doi.org/10.3390/cells11030401
Cho, Y.-D., Kim, K.-H., Lee, Y.-M., Ku, Y., & Seol, Y.-J. (2021). Periodontal wound healing and tissue regeneration: A narrative review. Pharmaceuticals, 14(5), 456.
Jamal, A. (2023). Embracing nature’s therapeutic potential: Herbal medicine. International Journal of Multidisciplinary Sciences and Arts, 2(3), 117–126.
Kalangit, R. B., & Octarina, O. (2024). Metode uji sitotoksisitas biomaterial dengan bentuk scaffold padatan dan berpori spons. Jurnal Kedokteran Gigi Terpadu (JKGT), 6(1), 50–52. https://doi.org/10.25105/jkgt.v6i1.20886
Manisha, D. R. B., Begam, A. M., Chahal, K. S., & Ashok, M. A. (2025). Medicinal plants and traditional uses and modern applications. Journal of Neonatal Surgery, 14(3), 162–175.
Mazumder, M. A. R., Tolaema, A., Chaikhemarat, P., & Rawdkuen, S. (2023). Antioxidant and anti-cytotoxicity effect of phenolic extracts from Psidium guajava Linn. leaves by novel assisted extraction techniques. Foods, 12, 2336.
Michalczyk, E. R., Senderak, A. R., Jones, R. M., Coulter, W. H., & Goudy, S. L. (2022). Changes in the microbiome during oral wound healing. Dentistry Review, 2(1), 100040.
Nazliniwaty, N., & Laila, L. (2019). Formulation and antibacterial activity of Plectranthus amboinicus (Lour.) Spreng leaves ethanolic extract as herbal mouthwash against halitosis caused bacteria. Open Access Macedonian Journal of Medical Sciences, 7(22), 3900–3903. https://doi.org/10.3889/oamjms.2019.529
Procházková, D., Boušová, I., & Wilhelmová, N. (2011). Antioxidant and prooxidant properties of flavonoids. Fitoterapia, 82(4), 513–523.
Rorrong, A. G., Lira, M. P., & Sondakh, J. J. (2024). Uji efektivitas antiinflamasi sediaan hidrogel daun miana (Coleus scutellarioides [L.] Benth.) secara in vivo. Jurnal Kesehatan Tambusai, 5(3), 8007–8017.
Shah, R., Domah, F., Shah, N., & Domah, J. (2020). Surgical wound healing in the oral cavity: A review. Dental Update, 47(2), 135–143.
Smith, P. C., Martínez, C., Martínez, J., & McCulloch, C. A. (2019). Role of fibroblast populations in periodontal wound healing and tissue remodeling. Frontiers in Physiology, 10, 270.
Sumbayak, E. M. (2015). Fibroblas: Struktur dan peranannya dalam penyembuhan luka. Fakultas Kedokteran, Universitas Kristen Krida Wacana.
Ubaedilah, N. A., & Supriyatna, A. (2023). Analisis dan penerapan manfaat kandungan senyawa daun miana (Coleus scutellarioides (L.) Benth.) di Kiaracondong, Kota Bandung. Hippocampus: Jurnal Pengabdian Kepada Masyarakat, 2(1), 75–82.
Villegas-Aguilar, M. D. C., Sánchez-Marzo, N., Fernández-Ochoa, Á., Del Río, C., Montaner, J., Micol, V., Herranz-López, M., Barrajón-Catalán, E., Arráez-Román, D., Cádiz-Gurrea, M. L., & Segura-Carretero, A. (2024). Evaluation of bioactive effects of five plant extracts with different phenolic compositions against different therapeutic targets. Antioxidants, 13(2), 217.
Vu, H. T., Yoon, J.-Y., Park, J.-H., Lee, H.-H., Dashnyam, K., Kim, H.-W., Lee, J.-H., Shin, J.-S., & Kim, J.-B. (2022). The potential application of human gingival fibroblast-conditioned media in pulp regeneration: An in vitro study. Cells, 11(21), 3398. https://doi.org/10.3390/cells11213398
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